β-glucan, homopolymers of glucose, are found abundantly in various microorganisms, mushrooms, and plants. These β-glucan are of the high value of attention because of their bioactivity and medicinal benefits. Multiple studies show that β-glucan have the potential to enhance the immune system. However, the β-glucan from different sources have different modulating effects. It depends upon the primary chemical structure of β-glucan. One crucial source of β-glucan is yeast, especially baker’s yeast (Saccharomyces cerevisiae). The yeast-derived β-glucan, (1,3)-β-linked with small chains of (1,6)-β-linked side chains, are known for their immune-modulating effect (Chan et al., 2009).  The extraction of yeast β-glucan is an

The beta-glucan derived from different sources have different molecular structures, which result in different immune activities. For example, the yeast beta1,3/1,6-D-glucanase has a unique molecular structure, especially the ratio of beta-1,6-glycosidic bonds to the total number of glycosidic bonds. This molecular structure makes it easy to recognized quickly by immune cell receptors, which results in efficient immune cell regulation (Danielson et al., 2010). Mainstream testing methods in the world It is important to test the quality of beta-glucan in yeast. Usually, the contents of beta-glucan determined the quality of yeast beta-glucan. Generally, the beta-glucan with high content has better quality.